FIGURE

Fig. 10

ID

ZDB-FIG-260407-39

Publication

Chojnacki et al., 2026 - Click-to-Release Reactions for Tertiary Amines and Pyridines

Other Figures

All Figure Page

Back to All Figure Page

Fig. 10

Validation of prodrug activation in cell-based assays. (a) Activation of the TzMe-prodrug of auristatin E (TzMe-AurE) by tert-butyl isonitrile (tBuNC). (b) Viability of MIAPaCa-2 cells in the presence of increasing concentrations of TzMe-AurE, auristatin E, and TzMe-AurE coincubated with tBuNC (600 μM). (c) EC50 values for auristatin E, TzMe-AurE, and TzMe-AurE coincubated with tBuNC. (d) Activation of the TzMe-prodrug of fentanyl (TzMe-Fentanyl) by tBuNC. (e) Dose–response curves for TzMe-fentanyl, fentanyl, and the corresponding samples preincubated with tBuNC (800 μM, 24 h, 37 °C) in a TRUPATH μ-opioid signaling assay. (f) EC50 values for fentanyl, TzMe-Fentanyl, and TzMe-fentanyl preincubated with tBuNC.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Am. Chem. Soc.