Figure 5

Prognostic evaluation of MFNG expression in colorectal cancer. (A) ROC curves of nine machine-learning classifiers in the training cohort. (B) ROC curves of the same classifiers in the independent validation cohort. (C) Feature importance ranking by L1-regularized logistic regression. (D–G) Kaplan–Meier curves for overall survival in GSE39582, GSE72970, GSE87211 and TCGA cohorts. (H and I) Kaplan–Meier curves for disease-free survival in GSE161158 and TCGA cohorts. (J and K) Kaplan–Meier curves for disease-specific survival in GSE87211 and TCGA cohorts. (L) Kaplan–Meier curve for progression-free survival in the TCGA cohort. (M) Box-plots comparing MFNG expression between tumor and normal tissuesin TCGA database. (N–T) Association between MFNG expression and (N) sex, (O) age, (P) recurrence, (Q) stage, (R) T, (S) N, (T) M in TCGA database. (U) Gene Set Enrichment Analysis (Hallmark) of high versus low MFNG expression tumors. (V) Comparison of somatic mutation frequencies between high- and low-MFNG groups. ***, p < 0.001.