Fig. 4

RABEP1 is crucial for the motility of HL-60 cells. (A, B) Representative images and quantification of immunoblots for RABAPTIN in control shRNA- and RABEP1 shRNA-expressing dHL-60 cells. TUBULIN serves as a loading control. The immunoblot was performed with three biological repeats, and quantification is shown as mean ± SD, using Dunnett's multiple comparisons test. (C, D) Representative tracking images and quantification of the random migration of control shRNA or RABEP1 shRNA-expressing dHL-60 cells under agarose. Scale bar, 100 μm. The assay was conducted with three biological repeats, with 31-58 dHL-60 cells tracked per group. Data are presented as mean ± SD, analyzed with Dunnett's test. (E, F) Representative images and quantification of RABAPTIN immunoblots in control shRNA with GFR control rescue, RABEP1 shRNA rescued with GFP control, full-length (FL) or Δ5-2 RABAPTIN in dHL-60 cells. TUBULIN was used as a loading control. The immunoblot included three biological repeats, and quantification is presented as mean ± SD, with Dunnett's test. (G, H) Representative images and quantified data of tracked random migration under agarose in the upper described groups. Scale bar, 100 μm. The assay involved three biological repeats, each tracking 24-48 dHL-60 cells per group. Data are shown as mean ± SD, analyzed with Dunnett's multiple comparisons test.