Figure 5

Spatial expression of nrg3b in the adult zebrafish telencephalon. A, The schematic view of the adult zebrafish forebrain shows the telencephalon’s major subregions in a coronal section. Areas corresponding to the dorsal (Dd, Dm, Dp) and ventral (Vd, Vv) domains of the telencephalon are indicated. Adapted conceptually from the telencephalic atlas published by (Diotel et al., 2015). B, ISH on coronal brain sections revealed expression of the nrg3b gene predominantly in the dorsomedial (Dm), ventral (Vd), and ventro-ventral (Vv) nuclei of the telencephalon. Scale bars: 50 μm. Stained tracts and nuclei overlapped with regions composed of rich populations of neurons and glial cells, which are involved in adult neurogenesis and the organization of neural circuits. The Lot was visible and served as an anatomical reference. No staining was observed in negative-control sections processed without probe (NC), confirming the specificity of the signal.