FIGURE

Figure S5.

ID

ZDB-FIG-260105-56

Publication

Li et al., 2026 - Stage- and tissue-specific gene editing using 4-OHT-inducible Cas9 in whole organism

Other Figures

All Figure Page

Back to All Figure Page

Figure S5.

4-OHT dosing selection for embryos and adults. (A) Schematic showing the 4-OHT treatment strategy on embryos. (B) Table showing survival rates of embryos treated with different concentrations of 4-OHT from the sphere stage to 2 dpf. (C) Gel electrophoretic image showing the T7E1 assay outcome of the tbx16 locus. The mutant band was indicated by the arrow. NC, negative control. (D) Immunostaining of Cas9 (magenta) and nuclei stained by DAPI (blue) in PGCs within the DMSO or 40 μM 4-OHT–treated embryos at 2 dpf. (E) Schematic showing the 4-OHT treatment strategy on 45 dpf females and detection of spawning rates. (F) Bar chart showing the spawning rates of adults treated with 10 and 5 μM 4-OHT at 45 dpf. Adults: The serial number of treated transgenic zebrafish. Source data are available for this figure: SourceData FS5.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Biol.