Exclusion of Sorbs1 exon 25 in mice skeletal muscle affects NMJ stability. (a) Schematic representation of the U7—Sorbs1 exon 25 antisense constructs (U7‐ESE25) and the experimental protocol. (b) RT‐PCR analysis and quantification of Sorbs1 exon 25 inclusion in TA muscles injected with AAV‐U7‐ESE25 compared with contralateral TA muscles injected with PBS (Ctrl) 2 months (p < 0.0001, N = 5 mice, Student's t‐test) and 6 months post injection (p < 0.001, N = 5 mice, unpaired Student's t‐test). (c) Representative immunofluorescence from confocal Z projections of NMJ from TA muscles injected with AAV‐U7‐ESE25 compared with contralateral TA muscles injected with PBS (Ctrl) 2 months post injection. (d) Violin plots of nerve terminal area and AChR area output using NMJ‐Morph. Ctrl TA (n = 57) from N = 5 mice; U7‐treated TA (n = 54) from N = 5 mice. (e) Representative immunofluorescence from confocal Z projections of NMJ from TA muscles injected with AAV‐U7‐ESE25 compared with contralateral TA muscles injected with PBS (Ctrl) 6 months post injection. (f) Violin plots of nerve terminal area and AChR area output using NMJ‐Morph. Ctrl TA (n = 234) from N = 6 mice; U7‐treated TA (n = 327) from N = 6 mice. p < 0.01 and p < 0.05, unpaired Student's t‐test.
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