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FIGURE 2

ID

ZDB-FIG-251115-147

Publication

Shinkai et al., 2025 - Refinement of the novel tank diving test: toward standardized and robust analysis of anxiety-like behavior in zebrafish

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FIGURE 2

Optimization of entry and freezing detection after tracking. (A) Determination of the optimal region-entry time threshold (1.11 s) for defining valid entries into the upper half of the tank based on F-score analysis. This analysis was conducted using 82,887 frames collected from seven females and 98,420 frames from seven male zebrafish. (B) Comparison of manual observation and software-based freezing detection. Solid lines and bars indicate manual annotation and software classification, respectively (blue: swimming; red: freezing). (C,D) Histogram of horizontal (C) and vertical (D) displacements during manually confirmed freezing frames, indicating Gaussian-distributed noise. (E) Instantaneous and average velocities were plotted on a logarithmic scale. Both clusters can be approximately separated with the axis y = x. For the analysis in B–E, freezing behavior was analyzed using 10,488 frames (in red) collected from one male and one female fish, while swimming behavior was analyzed using 3,217 frames (in blue) collected from three different males and one female fish.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Behav. Neurosci.