Fig. 3

Cfap298ΔΔS mutants develop normal nodes. (A) Representative immunofluorescent images of E8.5 nodes from wild-type and Cfap298ΔΔS mutant embryos. Embryos are stained for cilia with acetylated tubulin (green) and γ-tubulin (magenta) antibodies. For wild-type and mutant nodes, one plane is shown for the crown cells and another plane is shown for pit cells along with a z-projection of the whole node. (B) Images of cilia are shown for wild-type and mutant nodes. Quantifications of cilium length for wild-type (n=3 nodes, 150 cilia total) and mutant (n=3 nodes, 150 cilia total) embryos showing no significant difference (P=0.76 by unpaired t-test). (C) Images showing CELSR1 (cyan) and γ-tubulin (red) marking basal bodies in wild-type (+/+) and Cfap298ΔΔS mutant embryos. Boxes in i and ii indicate regions shown in images iii and iv, respectively. (D) Circular histograms display magnitude and orientation of CELSR1 polarity along the AP axis of wild-type (n=3 nodes, 99 cells total) and mutant (n=3, 218 cells total) embryos. Average polarity magnitudes from wild-type and mutant nodes were determined not to be significantly different (P=0.0691 by unpaired t-test). (E) Quantification of basal body polarity along the AP axis of the wild-type (n=3, 86 cells total) and mutant (n=3, 97 cells total) embryos showing no significant difference (P=0.2877 by Chi-squared test). ns, not significant (P>0.05). Scale bars: 10 μm.