Fig. 2

Cfap298ΔΔS mutants have perturbed left–right patterning. (A–B‴) Representative HCR images for Nodal (green), Pitx2 (magenta) and Lefty1 (yellow) expression in a whole-mount E8.5 wild-type embryo (A) and Cfap298ΔΔS mutants (B′–B‴). Wild-type embryos exhibit left-sided Nodal and Pitx2 expression in the lateral plate mesoderm (LPM) (arrowheads) with Lefty1 expression along the embryonic midline (asterisk). (B–B‴) Images from Cfap298ΔΔS mutants showing one example of right-sided Nodal, Lefty and Pitx2 expression along the LPM (arrowheads) (B′), an example where Nodal and Pitx2 are absent in the LPM and Lefty1 is expressed weakly at the midline (B″, asterisk), and an example of abnormal expression with reduced and bilateral Nodal, right-sided Pitx2 (arrowheads) and absent Lefty1 in the midline (asterisk) (B‴). Scale bars: 100 μm. (C) Diagram of an E8.5 wild-type embryo viewed from the dorsal side, anterior is up. Expanded view of a cross-section through the node is shown on the right. Representative images of E8.5 node labeled by HCR for Nodal (green), Lefty1 (yellow) and Cfap298 (magenta) transcripts. Cross-section (1) and planar (2 and 3) views of the node showing Nodal expression in the crown cells but not at the pit cells. Lefty1-expressing cells mark the floorplate of the neural tube are visible at the base of the pit of the node. Cfap298-expressing cells are visible throughout the node. Scale bars: 10 μm.