sgms2a and sgms2b mRNA expression in WT zebrafish embryos and early larvae by whole-mount in situ hybridization and RT-qPCR. (A) Spatial expression pattern of sgms2a mRNA at 1, 2, 3, and 6 dpf WT zebrafish by whole-mount in situ hybridization. Expression-specific structures are indicated with abbreviations. sgms2a negative control is presented in Figure S1. (B) Spatial expression pattern of sgms2b mRNA at 1, 2, 3, and 6 dpf WT zebrafish by whole-mount in situ hybridization. Expression-specific structures are indicated with abbreviations. sgms2b negative control is presented in Figure S1. (C-E) Expression patterns of sgms2a and sgms2b (relative expression to rpl13a) at 1, 2, 3, 5, and 7 dpf WT whole-body zebrafish by RT-qPCR. In (C) and (D), expression at each time point was compared to the expression at time point 1 dpf (expression at 1 dpf was set to 1 (shown as dotted line)). In (E), sgms2b and sgms2a expression was compared (the expression of sgms2a at each time point was set to 1 (shown as dotted line). Data are shown as scatter dot plots with mean ± SD. The p-values were determined using an unpaired t-test. The results from three independent experiments were combined. p < .05 was considered statistically significant. Only significant p-values were presented. bb, basibranchial; br, brain; cb, ceratobrancial; ch, ceratohyal; cl, cleithrum; dpf, days post-fertilization; hb, hypobranchial; m, Meckel’s cartilage; myo, myotome; o, opercle; ov, otic vesicle; ps, parasphenoid. The whole-mount in situ hybridization experiments were performed twice (n = 5-10 embryos or larvae/time point/experiment). Zebrafish orientation in both (A) and (B): upper row from left to right: lateral, lateral, dorsal; lower row from left to right: lateral, dorsal, dorsal. Scale bar = 200 μm.
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