FIGURE

Fig. 5

ID

ZDB-FIG-251022-64

Publication

Tran et al., 2025 - Redundant and novel functions of scube genes during zebrafish development

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Fig. 5

Increased apoptosis and reduced macrophage numbers in scube mutants. (A) The TUNEL assay revealed a greater number of apoptotic cells in scube1−/−, scube3−/−, and scube1−/−scube3−/− compared with wild-type at 30 hpf. Double mutants for scube1 and scube3 showed significantly higher numbers of apoptotic cells than either single mutant. ANOVA F(3,24) = 107.9, p < 0.0001. (B) Macrophage numbers decrease in scube mutants. Tg(mpeg1:Gal4-FF)gl25/Tg(UAS-E1b:nfsB.mCherry)c264 reporter line was used to label macrophages. Live imaging of zebrafish at 3 dpf showed fewer macrophages in scube1−/−, scube3−/−, and the double mutant scube1−/−scube3−/− compared to controls. The double mutant displayed a further reduction in macrophage numbers compared to either single mutant. ANOVA, F (3, 22) = 41.26, p < 0.0001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	scube1^pc61/pc61 scube3^pc60/pc60 scube1^pc61/pc61; c264Tg; gl25Tg scube1^pc61/pc61; scube3^pc60/pc60 scube3^pc60/pc60; c264Tg; gl25Tg scube1^pc61/pc61; scube3^pc60/pc60; c264Tg; gl25Tg
Observed In:	whole organism apoptotic process whole organism macrophage
Stage Range:	Prim-15 to Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 528, Tran, Q.D., Mirkovic, I., Miles, L.B., Berger, J., Wood, A.J., Ruparelia, A.A., Shehni, S.A., Currie, P.D., Redundant and novel functions of scube genes during zebrafish development, 79-90, Copyright (2025) with permission from Elsevier. Full text @ Dev. Biol.