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Fig. 5 Increased apoptosis and reduced macrophage numbers in scube mutants. (A) The TUNEL assay revealed a greater number of apoptotic cells in scube1−/−, scube3−/−, and scube1−/−scube3−/− compared with wild-type at 30 hpf. Double mutants for scube1 and scube3 showed significantly higher numbers of apoptotic cells than either single mutant. ANOVA F(3,24) = 107.9, p < 0.0001. (B) Macrophage numbers decrease in scube mutants. Tg(mpeg1:Gal4-FF)gl25/Tg(UAS-E1b:nfsB.mCherry)c264 reporter line was used to label macrophages. Live imaging of zebrafish at 3 dpf showed fewer macrophages in scube1−/−, scube3−/−, and the double mutant scube1−/−scube3−/− compared to controls. The double mutant displayed a further reduction in macrophage numbers compared to either single mutant. ANOVA, F (3, 22) = 41.26, p < 0.0001.
Reprinted from Developmental Biology, , Tran, Q.D., Mirkovic, I., Miles, L.B., Berger, J., Wood, A.J., Ruparelia, A.A., Shehni, S.A., Currie, P.D., Redundant and novel functions of scube genes during zebrafish development, , Copyright (2025) with permission from Elsevier. Full text @ Dev. Biol.