Social interaction and aggressiveness in WT and ube3a mutant zebrafish. (a) Schematic representation of the social interaction test. (b) Representative trajectory diagrams of WT (left) and mutant (right) zebrafish during the social interaction test. (c) Percentage of time spent near conspecifics for WT (N = 12) and mutants (N = 12) ube3a. (d) Schematic representation of the kin preference test. (e) Representative trajectory diagrams of WT (left) and mutant (right) zebrafish during the kin preference test. (f) Percentage of time spent near non-kin individuals (left), and near kin individuals (right) for WT (N = 12) and mutants (N = 11) ube3a. (g) Schematic diagram of the shoaling test. (h) Mean distance to neighbors (left) and proportion of time spent with at least one neighbor (right) between WT (N = 12) and mutants (N = 12). (i) Representative trajectory diagrams of WT (top) and mutant (bottom) zebrafish in the mirror attack test (aggression test). (j) Number of attacks directed at the mirror (left), average speed (middle), and distance traveled during the mirror test (right) for WT (N = 12) and mutants (N = 11) ube3a. The data are presented as the mean ± SEM. ns: non-significant, **P < 0.01, ***P < 0.001 and ***P < 0.0001. Schematic representations were created with BioRender.com.
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