BEC-Laminin Interactions are Required for Efficient Wound Closure in the Periderm. A Representative live confocal images of Tg(krt5:Gal4ff)la212; Tg(UAS:LifeAct-GFP)mu271 for LifeAct-GFP under SEC-specific krt5 promoter, showing the central and peripheral regions of the fin fold at 2dpf before and after single-cell ablation. Shaded border indicates the ablated cell and subsequent wound closure. B Quantification of time (min) required for complete wound closure. Data presented as box plots with whiskers to minimum and maximum value. Bounds mark the 25th and 75th percentiles. Central line is the median. (two-tailed Mann-Whitney test, p = 0.0040, n = 8 (Center) and 6 (Periphery) embryos; 4 independent experiments). C Representative live confocal images of Tg(krt5:Gal4ff)la212; Tg(UAS:LifeAct-GFP)mu271 for LifeAct-GFP under the SEC-specific krt5 promoter in the peripheral regions of fin fold of indicated genotypes at 2dpf before and after single-cell ablation. Shaded border indicates the ablated cell and subsequent wound closure. D Quantification of periphery wound closure time (min), comparing WT and dspa+/- mutants. Data presented as box plots with whiskers to minimum and maximum value. Bounds mark the 25th and 75th percentiles. Central line is the median. (Mann-Whitney test, p = 0.7879, n = 6 (WT) and 5 (dspa+/-) embryos; 4 independent experiments). E Quantification of wound closure time (min), comparing periphery WT, lama5+/-, and lama5+/-, dspa+/- mutants. Data presented as box plots with whiskers to minimum and maximum value. Bounds mark the 25th and 75th percentiles. Central line is the median. (Kruskal-Wallis test with multiple comparisons; p = 0.0273 (WT vs. lama5+/-), > 0.9999 (WT vs. lama5+/-, dspa+/-), and 0.1988 (lama5+/- vs. lama5+/-, dspa+/-); n = 6 (WT), 9 (lama5 +/-), and 5 (lama5+/-, dspa+/-) embryos; 5 independent experiments. Source data are provided as a Source Data file.
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