FIGURE

Fig. 3

ID

ZDB-FIG-251022-31

Publication

He et al., 2025 - Epidermal stem cells control periderm injury repair via matrix-driven specialization of intercellular junctions

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Fig. 3

BEC-ECM Adhesions Regulate the Balance Between Desmosome and Cadherin Junctions Across Layers. A Representative image of wild type (WT) vs. itgb1a+/-, itgb1b+/- fin fold, stained with a FAM-tagged collagen hybridizing peptide and Laminin-A1 at ~48hpf. Intensity displayed using fire LUT. B Quantification of normalized collagen and laminin intensity across fin fold (edge = 0 µm). Each curve is normalized to individual embryo maximum. C Quantification of average normalized collagen and laminin intensity/embryo (Collagen: Ordinary One-Way ANOVA test with multiple comparisons, p = 0.6422 (Center) and 0.4573 (Periphery), n = 18 (WT) and 27 (itgb1a+/-, itgb1b+/-) embryos; 3 independent experiments; Laminin: Kruskal-Wallis test with multiple comparisons, p > 0.9999, n = 6 (WT) and 11 (itgb1a+/-, itgb1b+/-) embryos; 2 independent experiments. D, F,H, J Representative images of cdh1-tdTomatoxt18 ((D) center and (F) periphery and immunofluorescence staining for desmoplakin (H) center and (J) periphery in zebrafish fin folds at 48hpf. Images show SECs, Interlayer (SEC-BEC), and BECs. Z-stack images were taken from the SEC-BEC layer. SECs and BECs were identified by maximum projection of selected z slices based on their z-position. Representative interlayer images are shown in orthogonal view of single z slice. 16-color intensity scale. E, G, I, K Quantification of E-cadherin (E) center and (G) periphery and desmoplakin (I) center and (K) periphery at SEC junctions, interlayer, and BEC junctions shown as violin plot (distribution=individual junctional measurements, dots= average values/embryo). E, G For all graphs, E-cadherin intensity was normalized to the WT Center BEC group (Violin plots tested for two-tailed unpaired t test (Center Interlayer) and two-tailed Mann-Whitney test for all other comparisons, p < 0.0001 (all comparisons), n = 180−200 junctions (SEC and BEC junctions) and 90–100 cells (interlayer), dots n = 9 (WT) and 10 (itgb1a+/-, itgb1b+/-) embryos; 2 independent experiments). I, K For all graphs, desmoplakin intensity was normalized to the WT Center BEC group (Violin plots tested for two-tailed Mann-Whitney test, p < 0.0001 (all comparisons), n = 359 − 395 junctions (SEC and BEC junctions) and 188−190 cells (interlayer), dots n = 20 (WT) and 19 (itgb1a+/-, itgb1b+/-) embryos; 2 independent experiments). Source data are provided as a Source Data file.

Expression Data

Gene:	Tomato
Antibodies:	Ab1-dsp Ab1-lama1
Fish:	cdh1^{xt18Tg/xt18Tg} itgb1a^sk109/+; itgb1b^sk110/+ itgb1a^sk109/+; itgb1b^sk110/+; cdh1^{xt18Tg/xt18Tg}
Anatomical Terms:	caudal fin epidermal basal stratum epidermal basal stratum central region epidermal basal stratum peripheral region epidermal superficial stratum central region epidermal superficial stratum peripheral region
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	itgb1a^sk109/+; itgb1b^sk110/+ itgb1a^sk109/+; itgb1b^sk110/+; cdh1^{xt18Tg/xt18Tg}
Observed In:	epidermal basal stratum central region epidermal basal stratum collagen fibril organization epidermal basal stratum peripheral region epidermal superficial stratum central region epidermal superficial stratum peripheral region
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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