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Enrichment of mesenchymal genes in snai1b+ CMs. a, b The CMs and SMs were isolated from a single-cell RNA seq dataset of 5-dpf hearts. These cells produced a sub-dataset with three clusters. snai1b+ CMs (green squares) were found in a cluster with a mixture of CMs and SMs, resembling the BV annulus region. The color scale indicates the normalized log counts of genes. The number of cells in the complete dataset was 366 after filtering, and the sub-dataset contains 53 cells. c, d Gene Ontology (GO) enrichment analysis categorized the enriched Biological Processes (BP) and Molecular Functions (MF). The BV annulus CMs had enriched mesenchymal BPs and MFs (blue and red boxes), compared to the ventricular CMs. The total number of marker genes selected for GO analysis was 151 for Ventricular CM and 147 for BV annulus CMs. The dot size indicates the gene ratio (i.e., # of genes in a category divided by # of total genes). The color scale depicts the Benjamini & Hochberg (1995) adjusted p-values. The original p-values are calculated using a hypergeometric test (one-tailed) via the enrichGO function in clusterProfiler. Top five enriched terms with p-values < 0.05 from each group are displayed. Source data are provided as a Source Data file. e, f Heatmaps showing the genes involved in each of the BP and MF categories for BV annulus CMs. The color scale indicates the log-fold change in mean expression for each gene in BV annulus CMs compared to ventricular CMs. Top five enriched terms with p-values < 0.05 are displayed. Source data are provided as a Source Data file.
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