Fig. 5

ISR inhibition reduces proinflammatory and migratory pathways. BMDMs from male 8-week-old C57Bl/6J mice were unpolarized (M0) or polarized to M1-like proinflammatory macrophages with and without 50 nM ISRIB and subjected to RNA-seq. n = 3 biological replicates. A PCA plot of RNA-seq results from vehicle- or ISRIB-treated unpolarized or M1-polarized BMDMs; n = 3 biological replicates. B Shared and uniquely expressed genes identified between vehicle- and ISRIB-treated BMDMs during M0 to M1-like transition phase. Venn diagram generated using BioVenn. C Heatmap of ISR pathway associated gene expression values. D Volcano plot of differentially expressed genes in M0 to M1-like transition in ISRIB-treated group. E GO pathway analysis of differentially expressed genes in M0 to M1-phase exclusive to ISRIB group. F Volcano plot of differentially expressed genes in ISRIB treated M1-polarized BMDMs compared to M1-polarized vehicle treated group. G GO pathway analysis of differentially expressed genes in ISRIB treated M1-polarized BMDMs when compared to vehicle group