FIGURE

Fig. 1

ID

ZDB-FIG-250903-61

Publication

Lange et al., 2025 - Genetic compensation highlights the importance of neural cell adhesion molecule Ncam1 paralogs in balancing signaling pathways during zebrafish lateral line development

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Fig. 1

ncam1b knockdown and knockout leads to different phenotypes in the development of the lateral line system. (A–D) Lateral views of 48 hpf Tg(ClaudinB::lynGFP) (A) Wild type (WT) (B) n1b morphant, (C) n1b -/- PMSC and (D) n1b -/- Del. Embryos are oriented with rostral to the left and dorsal to the top. (A) At 48 hpf, primI (star) of WT reaches the tip of the tail and deposits 6 proneuromasts (L1-L6), with the last one having just been deposited (arrow). The migration distance was measured from the otic vesicle (ov) to primI. (B) ncam1b morpholino injection leads to reduced migration of a smaller primI and deposition of fewer proneuromasts. (C + D) n1b -/- PMSC and n1b -/- Del mutants phenocopy the WT phenotype, with primI reaching the tip of the tail at 48 hpt and depositing 6 proneuromasts (L1-L6). (E–G) Quantification of migration distance, primI length and number of proneuromasts in WT, ncam1b morpholino injected embryos, n1b -/- PMSC and n1b -/- Del. Bars indicate mean values of the medians of each experiment, with error bars representing standard error. Statistic was made using two-sided t-test. Scale bar 200 µm. Abbreviations: WT: Wild type; n1b -/- PMSC: ncam1b -/- Premature Stop Codon mutant; n1b -/- Del: ncam1b deletion mutant; MO: Morpholino; primI: primordium; pnm: proneuromast; ov: otic vesicle.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Eur. J. Cell Biol.