Figure 6

Interactive cross-species analyses of HemAtlas

A. Visualization of fibroblasts in selected datasets from zebrafish CHT [36], mouse FL [30], and human FL [6], respectively. B. Violin plots showing the expression of sparc/Sparc/SPARC and igfbp5b/Igfbp5/IGFBP5 in all cell types from zebrafish CHT, mouse FL, and human FL, respectively, with the fibroblasts highlighted in red. The Kruskal–Wallis test was used to calculate the statistical significance across all the cell types in each dataset (****, P < 0.0001). C. Left: co-expression of sparc and igfbp5b with fibroblast markers col1a2 and col5a1 is observed in zebrafish CHT fibroblasts (arrowheads) by FISH. Scale bar, 50 μm. Right: quantitative real-time PCR showing that Sparc/SPARC and Igfbp5/IGFBP5 are expressed in fibroblasts of mouse and human FL (n = 3). Data are presented as mean ± SEM. Statistical difference was determined by Student’s t-test (n.s., not significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001). D. GO enrichment analyses showing the conserved fibroblast function across three species. The GO terms related to the regulation of hematopoiesis are highlighted in red. The order of the GO terms is based on the value of −log₁₀ adjusted P value. E. Representative ligand–receptor pairs with the top 10 highest communication probabilities between fibroblasts as ligand-expressing cells and HSPCs as receptor-expressing cells in expanded hematopoietic organs of three species. The order of the ligand–receptor pairs is based on their communication probabilities. F. Visualization of the Mdk–Itgb1 interaction pair between HSPCs and fibroblasts in mouse FL spatial transcriptome datasets [40]. Spots are color-coded by cell types and scaled gene expression of Mdk and Itgb1, respectively. “Both” represents spots that include both fibroblasts and ECs. Circle represents a closer spatial relationship between HSPCs and fibroblasts. FISH, fluorescence in situ hybridization; PCR, polymerase chain reaction; SEM, standard error of the mean; Fib, fibroblast; HC, hematopoietic cell; EC, endothelial cell; Max, maximum; Min, minimum.