Fig. 2

Gper1 mutants do not show habituation deficits after treatment with estradiol. A) Homozygous gper1(−/−) mutants (n = 81 fish, purple) do not show impaired habituation relative to sibling controls (gper1(+/?) = gper1(+/−) and gper1(+/+), n = 222 fish, red). Rather, there is a slight suppression of responsiveness in the mutant group (arrows), indicating weakly increased habituation. Each dot is the probability of response to one stimulus. Lines are smoothed in time with a Savitzky–Golay filter (window = 15 stimuli, order = 2). B-E) No significant differences are observed in the responsiveness distributions for the naive response to the first 5 DF stimuli (B), during the first training block (C), or the vibration response (E), while a subtle but statistically significant decrease in responsiveness is observed in the trained response (D). Statistical significance was calculated using Mann-Whitney U test, * = P < .05. F) Cumulative mean difference (CMD) plot quantifying habituation performance of mutants relative to sibling controls, consistent with slightly increased habituation rate in mutant larvae.