Fig 2

fn1a^bns692 mutants also display a variable cardiac phenotype.

(A) Schematic showing the WT, natter/tl43c, and bns692 fn1a alleles. Exons (n = 46) are indicated by black boxes. The start codons are represented by a green triangle and the stop codons by a red octagon. (B)In situ hybridisation with the myocardial marker myl7 was performed on 24 hpf fn1a^bns692 mutant embryos and WT siblings from four single pair fn1a^bns692 heterozygous intercrosses. The proportion of embryos matching the image shown is indicated in the top right corner of each image. The remaining 5/49 mutant embryos displayed a linear heart tube phenotype as noted in (C). Dorsal views are shown with anterior to the top. (C) Quantification of the 24 hpf myocardial migration phenotype in fn1a^bns692 mutants and WT siblings shown in (B). (D) Quantification of the 100 hpf cardiac phenotype in fn1a^bns692 mutants and WT siblings from three single pair fn1a^bns692 heterozygous intercrosses. The numbers on each bar in (C) and (D) indicate the total number of larvae assessed. All p-values were calculated using chi-squared tests. Scale bars, 100 µm.