Fig. 3

Clustering of differentially expressed transcripts in adjacent stages. A There were 15 clusters with clear single-time effects for the transcripts that were significantly differentially expressed in two adjacent periods. The heatmap shows the changes of normalized FPKM values of 15 clusters, among which cluster 25 and cluster 7 were highly expressed transcripts in the oblong stage, cluster 28, cluster 10, and cluster 16 were highly expressed transcripts in the maternal period. Cluster 8, cluster 11, and cluster 24 indicate zygote activation to begin the high level of transcription. Cluster 1, cluster 13, and cluster 26 after the pharyngula stage began showed high levels of transcription. Cluster 9, cluster 6, cluster 14, and cluster 27 were highly expressed transcripts at the beginning of segment development. The line plot shows the trend line of the average FPKM value, and the oblong period is the critical period of the transition from maternal to zygote expression. B The enrichment threshold of GO level 2 items for 15 clusters were set to a P-value less than 0.05, but cluster 26 had no level 2 items with P-values less than 0.05. C The enrichment categories of GO level 4 in cluster 26 show that this period focuses on signal transmission and material transport between organs