PUBLICATION
High resolution of full-length RNA sequencing deciphers massive transcriptome complexity during zebrafish embryogenesis
- Authors
- Bo, J., Fang, W., Wang, J., He, S., Yang, L.
- ID
- ZDB-PUB-250605-13
- Date
- 2025
- Source
- BMC Biology 23: 155155 (Journal)
- Registered Authors
- Wang, Jing
- Keywords
- Embryogenesis, Long-read sequencing, Zebrafish, Zygotic genome activation
- MeSH Terms
-
- Embryo, Nonmammalian/metabolism
- Transcriptome*
- Embryonic Development*/genetics
- Gene Expression Regulation, Developmental
- Sequence Analysis, RNA*
- Zebrafish*/embryology
- Zebrafish*/genetics
- Animals
- Molecular Sequence Annotation
- PubMed
- 40468300 Full text @ BMC Biol.
Citation
Bo, J., Fang, W., Wang, J., He, S., Yang, L. (2025) High resolution of full-length RNA sequencing deciphers massive transcriptome complexity during zebrafish embryogenesis. BMC Biology. 23:155155.
Abstract
Background The zebrafish has significantly advanced our understanding of human disease and development, with nearly 70% of single-copy protein-coding genes conserved between the species. However, research on zebrafish is limited by gaps in existing genome annotations, which are primarily based on computational predictions and short-read sequencing data.
Results To address this issue, we employed the PacBio Sequel II platform to generate a time-series full-length transcriptome landscape of zebrafish embryogenesis, covering 21 time points from embryo to six days post-fertilization. Our analysis uncovered 2113 previously unannotated genes and 33,018 novel isoforms of previously annotated genes, substantially expanding the current zebrafish gene annotations. We verified these findings using various methods, including domain prediction, homology analysis, conservation analysis, transcript quantification with short-read RNA-seq, and promoter position information with H3K4me3 and CAGE-seq. Furthermore, we analyzed the dynamic expression of transcripts across the 21 developmental stages using next-generation sequencing data, identifying variable splicing events throughout these stages.
Conclusions Collectively, our study provides a high-resolution and significantly improved transcriptome annotation during zebrafish embryogenesis, offering a valuable resource for the zebrafish research community.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping