FIGURE

Figure 1

ID

ZDB-FIG-250627-45

Publication

Nguyen et al., 2025 - Cannabinoid Receptor 1 Regulates Zebrafish Renal Multiciliated Cell Development via cAMP Signaling

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Figure 1

Loss of cnr1 leads to reduction in both mature and progenitor MCC populations: (A) live imaging between different treatment groups between 24 and 72 hpf with the percentage of embryos with edema between 48–72 hpf (arrows indicate pericardial edema); scale bar = 200 μm; (B) 28 ss WT, cnr1 MO stained via WISH using the mature MCC marker odf3b and cetn4; scale bar = 50 μm; (C,D) number of odf3b⁺ and cetn4⁺ cells per nephron at 28 ss; (E) 24 ss WT, cnr1 MO stained via WISH using the progenitor MCC marker pax2a and jag2b; scale bar = 50 μm; (F,G) number of pax2a⁺ and jag2b⁺ cells per nephron at 24 ss. Data presented on graphs are represented as mean ± SD; **** p < 0.0001 (t-test).

Expression Data

Genes:	cetn4 cimap1b jag2b pax2a
Fish:	TU TU + MO2-cnr1
Knockdown Reagent:	MO2-cnr1
Anatomical Term:	ciliated cell
Stage Range:	20-25 somites to 26+ somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TU TU + MO2-cnr1
Conditions:	chemical treatment by environment: anandamide chemical treatment by environment: Methanandamide
Knockdown Reagent:	MO2-cnr1
Observed In:	ciliated cell nephron ciliated cell pericardium
Stage Range:	20-25 somites to Protruding-mouth

Phenotype Detail

Acknowledgments

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