Fig. 3

Inhibition of M-current (IM) by XE-991 increases nerve activity but does not alter features of N-methyl-d-aspartic acid (NMDA)-induced fictive locomotion in spinalized 4–5 days postfertilization (dpf) larval zebrafish. A–H: fictive locomotion induced by 200 µM NMDA recorded from motor nerves of 4–5 dpf spinalized larval zebrafish (n = 5) before and after inhibition of IM by 10 µM XE-991. A and B: representative traces of locomotor activity induced by 200 µM NMDA (A; control) and when 10 µM XE-991 is introduced to the bath (B). C: mean episode duration (P = 0.8125). D: mean motor burst duration (P = 0.8125). E: mean number of motor bursts per episode (P = 0.8125). F: mean locomotor frequency (P = 0.1250). G: mean peak amplitude of locomotor activity (P >0.9999). H: mean inter-burst interval duration (P = 0.1250). I and J: representative trace of recording where NMDA application does not generate locomotor activity (I; control), but 10 µM XE-991 enhances motor output (J) (n = 26). K–N: measures of overall activity recorded from motor nerves 30 s before and after bath application of 10 µM XE-991 to the recording chamber. K: peak amplitude of activity. L: area under the curve. M: spikes per second. N: standard deviation of signal. Individual animals before and after drug application are color-matched. Statistical analysis, Wilcoxon matched-pairs test. **P < 0.01; ****P < 0.0001.