Fig. 2

Pharmacological enhancement of M-current (IM) reduces spontaneous swimming activity in larval zebrafish. A: representative traces of total swimming activity from individual larvae recorded over 5 min from control (i) and XE-991 (ii) groups tested concurrently. B: representative traces of total swimming activity from individual larvae recorded over 5 min from control (i) and ICA-069673 (ii) groups tested concurrently. C–E: effects of pharmacological inhibition of IM by 10 µM XE-991 on amount of slow (C; 2–10 mm/s; P = 0.3434), fast (D; >10 mm/s; P = 0.3983), and total (E; P = 0.6639) spontaneous swimming activity recorded over 20 min. F–H: effects of pharmacological inhibition of IM by 10 µM XE-991 on average distance swam per minute during slow (F; P = 0.8340), fast (G; P = 0.4581), and total spontaneous swimming (H; P = 0.4707). I–K: effects of pharmacological enhancement of IM by 10 µM ICA-069673 on amount of slow (I; P = 0.0794), fast (J), and total (K) spontaneous swimming activity recorded over 20 min. L–N: effects of pharmacological enhancement of IM by 10 µM ICA-069673 on average distance swam per minute during slow (L), fast (M), and total spontaneous swimming (N). Statistical analysis, Mann–Whitney test for non-normally distributed data sets and unpaired t tests for normally distributed data sets. *P < 0.05; **P < 0.01; ****P < 0.0001; otherwise not statistically significant.