Figure 4

Ligase activity of Dr-tRNA-LC.A, urea-PAGE analysis of the products of the enzymatic reaction carried out by the Dr-tRNA-LC (full-length [FL] and truncated [TR] variant) on the tRNA exon halves substrate. All reactions were performed in three replicates. B, urea-PAGE analysis of the products of the enzymatic reaction carried out by the Dr-tRNA-LC (FL and TR variant) on the short RNA substrate. Control reaction (CTRL) contained the RNA substrate and Dr-Archease but did not contain Dr-tRNA-LC. “–Arch” lane is the reaction without Dr-Archease. L, RNA corresponding to the expected product of the reaction. RA denotes the lane with R263A mutant. A schematic representation of the substrate and product is shown on the right. All reactions were performed in four replicates, (C) quantification of the data in (B). All four data points are plotted with error bar representing the SD of the measurements. Dr-tRNA-LC, ligase complex from Danio rerio.