FIGURE

FIGURE 3

ID

ZDB-FIG-250609-49

Publication

Mesika et al., 2025 - Impaired Proteostasis is Linked to Neurological Pathology in a Zebrafish NGLY1 Deficiency Model

Other Figures

All Figure Page

Back to All Figure Page

FIGURE 3

Impaired ubiquitin‐mediated protein degradation in the adult ngly1^(−/−)fish brain. (A) Heat map presentation of RNA sequencing of zebrafish brains, red = downregulation and blue = upregulation, the scale indicates fold change values. (B, C) RT‐qPCR quantification of ube3a(B) and ubb(C) mRNA levels. ** T‐test p < 0.01, *** T‐test p < 0.001 (ngly1^(+/+)n = 3, ngly1^(−/−)n = 3, ube3a, ubb). (D) Western blot analysis of zebrafish brain with an antibody against poly‐ubb; β‐Actin was used as a loading control. (E) Quantification of western blot‐determined ubiquitinated protein levels. **T‐test p < 0.01 (ngly1^(+/+)n = 9, ngly1^(−/−)n = 9). Representative immunohistochemistry images of ngly1^(+/+)(F) and ngly1^(−/−)(G) zebrafish brains periventricular gray zone (PGZ) reacted with anti‐poly‐ubb antibody, (H) Percentage of ubiquitin deposit‐positive cells. ***T‐test p < 0.001 (N = 10 in each group). Red arrows indicate cells with ubiquitinated protein accumulation.

Expression Data

Genes:	ubb ube3a
Antibody:	Ab4-ubb
Fish:	AB ngly1^{zf3678/zf3678} (AB)
Anatomical Terms:	brain periventricular grey zone
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ngly1^{zf3678/zf3678} (AB)
Observed In:	brain periventricular grey zone
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Inherit. Metab. Dis.