IMAGE

FIGURE 3

ID

ZDB-IMAGE-250609-42

Genes

ubb
ube3a

Antibodies

Ab4-ubb

Publication

Mesika et al., 2025 - Impaired Proteostasis is Linked to Neurological Pathology in a Zebrafish NGLY1 Deficiency Model

All Figures

Figures for Mesika et al., 2025

Image

Figure Caption

FIGURE 3

Impaired ubiquitin‐mediated protein degradation in the adult ngly1^(−/−)fish brain. (A) Heat map presentation of RNA sequencing of zebrafish brains, red = downregulation and blue = upregulation, the scale indicates fold change values. (B, C) RT‐qPCR quantification of ube3a(B) and ubb(C) mRNA levels. ** T‐test p < 0.01, *** T‐test p < 0.001 (ngly1^(+/+)n = 3, ngly1^(−/−)n = 3, ube3a, ubb). (D) Western blot analysis of zebrafish brain with an antibody against poly‐ubb; β‐Actin was used as a loading control. (E) Quantification of western blot‐determined ubiquitinated protein levels. **T‐test p < 0.01 (ngly1^(+/+)n = 9, ngly1^(−/−)n = 9). Representative immunohistochemistry images of ngly1^(+/+)(F) and ngly1^(−/−)(G) zebrafish brains periventricular gray zone (PGZ) reacted with anti‐poly‐ubb antibody, (H) Percentage of ubiquitin deposit‐positive cells. ***T‐test p < 0.001 (N = 10 in each group). Red arrows indicate cells with ubiquitinated protein accumulation.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Inherit. Metab. Dis.