FIGURE

Fig. 4

ID

ZDB-FIG-250515-37

Publication

Bochen et al., 2025 - TRPM4 contributes to cell death in prostate cancer tumor spheroids, and to extravasation and metastasis in a zebrafish xenograft model system

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Fig. 4

Transient receptor potential melastatin‐4 (TRPM4) knockout (KO) reduces extravasation and metastatic burden of PCa cells in zebrafish xenograft model. (A) Representative stereomicroscopic fluorescence images of metastatic colonization of 2 day postfertilization (dpf) zebrafish larvae with endothelial reporter tg(Fli:GFP), after injection of DU145 cells (n = 50) and corresponding TRPM4 KO clones (both n = 40) stably expressing tdTomato. Scale bar indicates 50 μm. (B) Tumor foci (left), average foci size (middle) and overall tumor burden (right) were measured at day 3 postinjection. Foci number and size were determined by automatic segmentation of the tdTomato fluorescence intensity within the tail of the injected zebrafish larvae. Tumor burden was quantified as average tdTomato fluorescence intensity within the same region. Fifty larvae injected with DU145 cells and 40 larvae injected with each of the corresponding TRPM4 KO clones were analyzed. All individual values are plotted as dots in the graph. The median is shown as a red line, whiskers represent the interquartile range. Significance levels for data from both clones in B are given relative to DU145 cells. Differences between samples were analyzed using one‐way ANOVA followed by Dunnett's post‐hoc test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Mol. Oncol.