Fig. 1

Overview of Experiment Timeline and Tasks. (A) Top view and the fish point of view of the 2 choice discrimination task. The wells and where the food reward was distributed are labelled. (B) Brief overview of the stimulus displayed to the fish during the conditioned place preference task. The fish are initially presented the check and the grey stimulus. Whichever is least preferred is rewarded in conditioning trials, shown by the star symbol. Then the probe presents both stimuli to the fish and the preference is measured again. (C) Fish of all groups started with isolation on the first day and then went through an open field test (OFT), habituation, motivation test, and training on the first task (either conditioned place preference or 2-choice discrimination task). After a break fish went through habituation, motivation test, and the second task (whichever task was not completed first). The study design was counterbalanced where half of the fish began with the CPP task while the other half began with the 2-choice discrimination task.