Fig 2

Effects of CX2 treatment before pentylenetetrazol (PTZ)-induced seizures on the number of seizure-like behaviors and swimming activity.

Animals exposed to CX2 for 24 hours before Pentylenetetrazol (PTZ)- induced seizures. Groups included: PTZ control (n=10); CX2 treatment at 0.5 µM (T0.5; n = 9), 0.1 µM (T0.1; n = 10), and 0.05 µM (T0.05; n = 10). Animals were video-recorded during a 10-minute exposure to PTZ (15 mM). (A) The number of seizures was scored visually twice per larva, with loss of posture as the criterion for a complete seizure event. (B) Distance traveled and (C) velocity of each larva were quantified using EthoVision XT locomotion tracking software (Noldus, Wageningen, The Netherlands). Data are expressed as mean ± SEM. Statistical analyses were conducted using one-way ANOVA followed by Bonferroni correction for multiple comparisons. Differences were considered significant at p < 0.05.