Fig 1

Effects of the Tripeptide treatment before pentylenetetrazol (PTZ)-induced seizures on seizure-like behaviors and swimming activity.

Animals were exposed to the Tripeptide for 24 hours before to Pentylenetetrazol (PTZ)- induced seizures. Groups included: PTZ control (n=10); Tripeptide treatment at 25 µg/mL (T25; n=10); 10 µg/ml (T10; n=10); 5 µg/ml (T5; n=10). Animals were video-recorded during a 10-minute exposure to PTZ (15mM). (A) The number of seizures was scored by visual analysis twice per larva, with loss of posture serving as the criterion for a complete seizure event; (B) Distance traveled and (C) velocity for each larva were quantified using EthoVision XT locomotion tracking software (Noldus, Wageningen, The Netherlands). Data are presented as mean ± SEM. Statistical analyses were performed using one-way ANOVA, followed by the Bonferroni correction for multiple comparisons. Differences were considered significant at p < 0.05.