Figure 5

TRPV4 mediated Ca²⁺ signaling participates the regulation of LIPUS to inflammatory chondrocyte. A) Heatmap showing ion channel gene expression in all cells from the mouse ACLR model of PTOA. B) The express distribution of TRPV4 in UMAP plots. C) Immunohistochemical staining of TRPV4 in the mouse knee joint. Calcium transient relative fluorescence intensity of ROIs with LIPUS stimuli in control group D), in TRPV4‐knockdown group E), and in the TRPV4‐blocked group F). Quantification of the number G) (n = 8), the magnitude H) (n = 8) of calcium peaks, and the responsive rate of cells I) (n = 4). J) Immunofluorescence was used to detect the LC3‐puncta after the chondrocytes was co‐incubated with GSK205. Scale bar, 5 µm. K) Quantification of LC3‐puncta in chondrocytes (n = 4). WB L) and densitometry analysis of Collagen II M) and MMP‐13 N) protein expression of chondrocyte co‐incubated with GSK205 following LIPUS treatment (n = 3). Data are presented as means ± SD. Statistical analysis was performed using Student's t test. ****(P < 0.0001), ***(P < 0.001), **(P < 0.01), *(P < 0.05), ns (0.05 < P).