Fig. 7

DMH1-treated larvae have significantly more ampullary organs than DMSO controls. (A) Scatter plot showing median and interquartile range for the total number of ampullary organs on one side of the head in stage 45 sterlet larvae that had been treated for 20 hours from stage 36 (i.e., from hatching to approximately stage 38, just prior to the onset of ampullary organ development) with DMH1 (n=17) or DMSO as controls (n=12). DMH1-treated larvae have significantly more ampullary organs (p<0.0001; two-tailed Mann-Whitney test). Ampullary organs were counted after in situ hybridisation [ISH] for Cacna1d or Kcnab3; raw counts are provided in Supplementary file 3. (B) Scatter plots showing median and interquartile range for the number of ampullary organs in each individual ampullary organ field on one side of the head in stage 45 sterlet larvae that had been treated for 20 hr from stage 36 with DMH1 (n=17), versus with DMSO as controls (n=12). Raw counts are provided in Supplementary file 3. For the location of each field, see schematic in panel C (reproduced from Figure 5D). Scatter plots are grouped with differently coloured titles according to lateral line placode (LLp) origin, following Gibbs and Northcutt, 2004: blue, anterodorsal LLp origin (supraorbital and infraorbital fields); orange, anteroventral LLp origin (preopercular fields); green, otic LLp origin (dorsal otic field); pink, supratemporal LLp origin (supratemporal field). All fields have significantly more ampullary organs in DMH1-treated larvae (n=17) than in DMSO controls (n=12; two-tailed Mann-Whitney tests). Asterisks on plots represent p values: **, p≤0.01; ***, p≤0.001; ****, p≤0.0001. p values for all fields are <0.0001 except for the ventral supraorbital field (p=0.0074), anterior preopercular field (p=0.0002) and posterior preopercular field (p=0.0003). (C) Schematic of a stage 45 sterlet larval head. Ampullary organ fields are represented by coloured patches flanking the neuromast lines, which are represented as dotted lines. The different field colours indicate their lateral line placode origin (consistent with scatter plot titles in B). Abbreviations for ampullary organ fields: app, anterior preopercular; di, dorsal infraorbital; dot, dorsal otic; ds, dorsal supraorbital; ppp, posterior preopercular; st, supratemporal; vi, ventral infraorbital; vs, ventral supraorbital. Abbreviations for neuromast lines: io, infraorbital; m, middle; ol, otic; pop, preopercular; so, supraorbital; st, supratemporal. Abbreviations for anatomical landmarks: an, anterior naris; b, barbel; e, eye; m, mouth; op, operculum; ot, otic vesicle; pn, posterior naris; s, spiracle (first gill cleft).