FIGURE

Figure 1

ID

ZDB-FIG-250411-11

Publication

Huang et al., 2025 - X-linked myopathy with excessive autophagy: characterization and therapy testing in a zebrafish model

Other Figures

All Figure Page

Back to All Figure Page

Figure 1

Generation and validation of <italic>vma21</italic> mutant zebrafish.

(A) Schematic of the zebrafish (Danio rerio) vma21 gene. Mutations in exon 2 of vma21 were created using CRISPR-Cas9 gene editing. (B) Two mutant lines were generated: (1) a 1 base pair (bp) deletion and (2) a 14 bp deletion with a 21 bp insertion. A compound heterozygous line was also generated by crossing the two mutant lines together. (C) Representative western blot of Vma21 and b-actin (housekeeping protein) protein levels in both the vma21 1 bp deletion (vma21^Δ1/Δ1) and the 14 bp deletion with 21 bp insertion (vma21^{Δ14ins21/Δ14ins21}) homozygous mutants compared to their respective wild-type/heterozygous sibling controls at 5 days post fertilization. Vma21 is ~11 kDa in size and b-actin is ~42 kDa. (D) Quantitative analysis of Vma21 western blot normalized to b-actin loading controls shows that Vma21 protein levels are significantly reduced in both vma21 homozygous mutants (fivefold less) compared to their respective sibling controls. Each western blot had a total of three biological replicates. For each sample, n = 30 zebrafish were utilized. All values are shown as mean ± SEM. Significance at **P < 0.01; ***P < 0.001 (unpaired two-tailed t test). Source data are available online for this figure.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ EMBO Mol. Med.