Clmp knockdown of zebrafish caused gastrointestinal motility disorder. In vivo imaging of zebrafish digestive organ with fluorescent reporters and statistical analysis of all groups' average body lengths and gut lengths. (A) 0.2% Calcein solution for 10 min at 28.5°C. Arrowheads indicate intestine; control group, n = 12; control-MO group, n = 5; clmp-MO group, n = 16. (B) 20 μg/mL Nile Red solution for 16 h at 28.5°C (each group, n = 6). *p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant. All scar bars: 1000 μm. clmp-MO, clmp morpholino group; control group; control-MO, control morpholino group; untreated group.
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