FIGURE 5

Immunohistochemistry in control and crim1^−/− larvae that are homozygous for a 2 base pair deletion, c.339_340delCT p.Leu112Leufs*3, at 72 and 96 h post fertilization (hpf). Representative sections of eyes from control larvae (A, C, E, G) and crim1^−/− larvae (B, D, F, H) at 72 hpf stained with zl-1 (A, B), E-cadherin (C, D), zn-5 antibody (E, F) and at 96 hpf stained with zpr-1 (G, H). The lens fiber cells in the crim1^−/− larva stained with z-l1 are less homogenous compared to the lens from a control larva (A, B). The corneal epithelium showed mild clumping of cells in the crim1^−/− larva stained with E-cadherin (C, D) compared to a control larva. The retinal ganglion cell layer was thicker in the crim1^−/− larva stained with the zn-5 antibody than in a control larva (E, F). There was no obvious difference in the photoreceptors in crim1^−/− and control larvae stained with zpr-1 (G, H). (A–H) Larvae stained with Alexa Fluor 488 dye (green). (A′–H′) Larvae stained with DAPI (blue). (A′′–H′′) Merged images. Scale bars = 50 µm.