Fig. 1.

ccn2a is reduced in sof^b123 mutants and expression localizes to joints by 4 dpa. (A) Fins were amputated at 50% and harvested at 5 dpa. Amputation planes are denoted by a white dotted line. In situ hybridization was performed using antisense digoxygenin-labeled probe against ccn2a to measure relative gene expression (n=4 per treatment group, with three biological replicates). (B) Reduction of gene expression was quantified through qPCR in both wildtype and sof^b123mutants. Graph shows a mean±s.e.m. of three biological replicates fold difference (n=5 fins per replicate). A fold difference of 1 means no change from wild-type expression. Student's t-test (two tailed, unpaired) was used to assess significance with a P-value of 0.01. (C) Fins were amputated at 50% and harvested at 3 dpa, 4 dpa, and 5 dpa. Amputation planes are denoted by white dotted line. In situ hybridization was performed using antisense digoxygenin-labeled probe against ccn2a to measure relative gene expression (n=5 per treatment group, with three biological replicates). Scale bar: 200 µm.