Fig. 6.

Yap inhibition reduced segment length in regenerating fins. (A) Verteporfin (VP) and DMSO injected fins were calcein stained and measured for regenerate length, indicated by black dotted lines (amputation plane is identified by white dotted lines). Verteporfin or DMSO was injected into one side of the fin and compared to their uninjected side to calculate percent similarity (n=26 for each treatment) with two biological replicates. (B) Graph displays mean±s.e.m. of percent similarity and showed a significant decrease in regenerate length compared to DMSO (two tailed, unpaired Student's t-test P<0.0001). (C) Verteporfin and DMSO injected fins were calcein stained and measured for segment length indicated by white lines and arrows. Verteporfin and DMSO were injected into one side of the fin and compared to their uninjected side to calculate percent similarity (n=26 for each treatment). Insets identify individual segments, joints are indicated by white arrows. (D) Percent similarity was calculated and showed a significant decrease in segment length compared to DMSO (two tailed, unpaired Student's t-test P=0.0001). Scale bars: 200 µm for top images; 100 µm for bottom images.