MYO1B is a target of miR-367-3p, and miR-367-3p regulates the progression of glioma through MYO1B. a The target genes of miR-367-3p was predicted by miRDB website. b, c The expression levels of target genes were detected in mRNA levels by qRT-PCR assay after overexpression of miR-367-3p in Ln229 (b) and U251 (c) cells (P = 0.0128 and 0.0062, 0.0002 and 0.0072). d The expression levels of MYO1B were detected in protein levels by western blot assay after overexpression of miR-367-3p in Ln229 and U251 cells. e The potential binding site between miR-367-3p and MYO1B 3’UTR was predicted by StarBase. f Schematic diagram of luciferase reporter plasmids construction, named pGL3-promoter-MYO1B-WT and pGL3-promoter-MYO1B-MUT. g Luciferase reporter assay was performed to assess the interaction between MYO1B 3’UTR and miR-367-3p (P = 0.0004). h, i The overexpression efficiency of MYO1B when transfection with pcDNA3.1- MYO1B plasmids in Ln229 (h) and U251 (i) cells at mRNA levels by qRT-PCR assays (P = 0.0039 and 0.0212). j. The overexpression efficiency of MYO1B when transfection with pcDNA3.1- MYO1B plasmids in Ln229 and U251 cells at protein level by western blot assays (P = 0.0039 and 0.0212). k, l CCK-8 assay was used to evaluate cell proliferation and migration when co-transfection of miR-367-3p mimics and pcDNA3.1- MYO1B in Ln229 (k) and U251 (l) cells. m, n Transwell assays were used to evaluate cell migration when co-transfection of miR-367-3p mimics and pcDNA3.1- MYO1B in Ln229 (m) and U251 (n) cells (P = 0.0495 and 0.0034). *: P < 0.05, **: P < 0.01, ***: P < 0.01
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