FIGURE

Fig. 1

ID

ZDB-FIG-250212-37

Publication

Carrara et al., 2025 - Dynamic interplay of cNHEJ and MMEJ pathways of DNA double-strand break repair during embryonic development in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

Analysis of MMEJ- and cNHEJ-related transcripts levels and distribution throughout embryonic development. (A-C) RT-qPCR analysis in embryos from 0 hpf to 72 hpf of relative mRNA levels for genes related to the MMEJ pathway and resection (A-B) and for genes related to the cNHEJ pathway (C). Note that, due to high expression levels, lig3 is presented separately (A). Expression levels are expressed as ratios to housekeeping genes (see methods). Data are means from four independent experiments. Error-bars are s.e.m. (D-E) Whole mount in situ hybridization for the same genes as in (A-C) genes related to the MMEJ pathway and regulation of resection (D) or related to the cNHEJ pathway (E) in wildtype embryos. Note that we could not produce a probe for artemis. Lateral view presented for 16 hpf, 24 hpf and left column of 48 hpf. Dorsal view presented in right column of 48 hpf. fb: fin buds; g: gut; e: eye; ov: otic vesicle; mhb: midbrain-hindbrain boundary. Scale bars: 500 microns.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.