FIGURE

Fig. 3.

ID

ZDB-FIG-250122-54

Publication

Willoughby et al., 2024 - Abca4, mutated in Stargardt Disease, is required for cone outer segment structural integrity

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Fig. 3.

Morphology of COS, ROS and RPE in retinas of 2-month-old zebrafish. (A-F) Confocal z-projections of COS, ROS and RPE in retinas obtained from wild-type or abca4a^ca30/ca30, abca4b^ca33/ca33, abca4a^ca30/ca30;abca4b^ca33/ca33 and abca4a^ca31/ca31;abca4b^ca33/ca33 mutant zebrafish. (A) Green COS stained for Green Opsin (green; z=3 µm), (B) red COS stained for PNA (red; z=3 µm), (C) blue COS stained for Blue Opsin (cyan; z=3.4 µm), (D) UV cones expressing EGFP stained for GFP (violet; z=4.4 µm), (E) ROS stained for rhodopsin (white; z=1.9 µm) and (F) RPE expressing tdTomato stained for RFP (white, z=2.4 µm). Counterstaining for nuclei is shown in dark blue (DAPI). White arrow in F (wild-type panel) indicates distal RPE processes around UV COS (not visible). Encircled areas show examples of phagosomes in retinas of wild-type and abca4b^ca33/^ca33 fish. White brackets in wild-type panels A-D indicate COS. Yellow arrows indicate examples of non RPE (‘mystery’) nuclei in B and C. Scale bars: 10 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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