Fig. 6

Periostin (POSTN) knockdown profoundly impacts MPNST cell morphology and growth.

A The human MPNST cell lines JH2-002, St88, and S462 express POSTN, with variability in expression level between cell lines (see also Fig. S9A, S8A for POSTN expression in S462 cells). B siRNA-mediated knockdown of POSTN in MPNST cells reduces its expression at both mRNA and protein levels, while expression of integrin receptor subunits is largely unaffected (see also Fig. S9B, S8B for POSTN expression in S462 cells). C POSTN knockdown significantly reduces MPNST cell size, as quantified by cytoplasmic area, and drastically alters cytoskeletal architecture (n = 120 cells per condition, imaged after 48 h incubation with control (Ctrl) or POSTN siRNA). Data for St88 cells are shown. D POSTN knockdown impairs MPNST cell growth by significantly reducing both cell viability and proliferative capacity. Data for St88 cells are shown. Significance, *p ≤ 0.05, **p ≤ 0.005, ***p ≤ 0.0005, and ****p ≤ 0.0001. See Fig. S8A, S9A for experimental timeline.