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Fig. 1

ID

ZDB-FIG-250118-49

Publication

Cero et al., 2024 - Profiling the cancer-prone microenvironment in a zebrafish model for MPNST

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Fig. 1

Use of a tg(sox10:RFP);brca2^hg5/hg5;tp53^zdf1/zdf1 zebrafish model to isolate and analyze the cellular component of a cancer-prone microenvironment.

A The optic nerve pathway (ONP) is a cancer-prone site in brca2^hg5/hg5;tp53^zdf1/zdf1 zebrafish. B Zebrafish ONP cancers exhibit ubiquitous sox10 expression (brown chromogen). Asterisk, blood vessel containing sox10-negative erythrocytes; arrows, fragments of optic nerve. Ret, retina; ON, optic nerve. C, D Zebrafish carrying a sox10-RFP reporter construct (tg(sox10:RFP);brca2^hg5/hg5;tp53^zdf1/zdf1) develop RFP-expressing cancers. E Experimental design showing experimental cohorts and workflow for tissue collection and RNA isolation. RFP-positive cells are shown in red. Remaining areas shaded in gray are composed of RFP-negative cells. ^‡RNA was of insufficient quantity for RNAseq analysis. F RFP-positive and RFP-negative cell fractions were collected from isolated ONP tissues by fluorescence-activated cell sorting (FACS). FACS analysis of control (not shown) and precancerous ONP samples showed similar distributions of RFP-positive and RFP-negative cell populations. The full gating strategy, including the panels shown in (F), is in Fig. S1. G Principal component analysis of samples analyzed by RNA-seq.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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