Fig. 6

Hand2 promotes Pdgfra expression in mouse cardiac cells.

a, Violin plots showing Pdgfra expression in cardiac precursors of E8.25 WT and Hand2^−/− mutant hearts (data analyzed from GSE126128 (ref. ⁵⁶)). b, Violin plots showing Pdgfra expression in posterior second heart field cells of E8.25 WT and Hand2^−/− mutant hearts (data analyzed from GSE126128 (ref. ⁵⁶)). c, Schematic of knockdown and overexpression of Hand2 during mESC differentiation into cardiac cells. d, Relative mRNA levels of Hand2 and Pdgfra in Hand2 knockdown mESC-derived cardiac cells; error bars are mean ± s.e.m.; n = 3 biologically independent samples. e, Relative mRNA levels of Hand2 and Pdgfra in Hand2 OE mESC-derived cardiac cells; error bars are mean ± s.e.m.; n = 3 biologically independent samples. P values in d and e were calculated using a one-way ANOVA multiple-comparison test. The average mRNA level in mESCs was set at 1.0. The C_t values of qPCR data are listed in Supplementary Table 1. Ctrl, control; KD, knockdown.