Fig. 4

Drug interaction scores [represented as windowed excess over Bliss (wEOB), x-axis] between neomycin and other drugs screened for ototoxic drug interactions, indicated in y-axis. wEOB is calculated for each well exposed to both drugs using a similar equation as excess over bliss (EOB) metric [11], with the formula EOB = Ra × Rb -Rab , where Ra and Rb are the responses of the individual drug dose exposures, and Rab is the response of the combined drug dose exposure. Wells with expected and observed responses outside 10%–90% were excluded from the aggregated calculation of the score across dose combinations for a particular drug pair, and the remaining data were averaged to yield the composite wEOB interaction score, intended to represent the overall trend of interaction between two drugs across a range of dose combinations.