Fig. 5

Compound B does not cause decreased glomerular filtration in AB zebrafish A.-I. Representative images of control and experimental compound treated AB zebrafish embryos showing red fluorescent dye distribution at 1, 5, and 24 hpi (A., dextran control-1 hpi; B., 2 µM cisplatin-1 hpi; C., 0.6 µM compound B-1 hpi; D., dextran control-5 hpi; E., 2 µM cisplatin-5 hpi; F., 0.6 µM compound B-5 hpi; G., dextran control-24 hpi; H., 2 µM cisplatin-24 hpi; I., 0.6 µM compound B-24 hpi; ), J. Examples of region-of-interest (ROI) analysis (top panel: representative polygon for ROI measurement of fluorescence; bottom panel: representative polygon for ROI measurement of background), K., Graph of fluorescent intensity for control and experimental compound treated zebrafish embryos at 1, 5, and 24 hpi. Key: CTCF (RFU) = corrected total cell fluorescence in relative fluorescence units; white columns = dextran control; light gray columns = 2 µM cisplatin; dark gray columns = 0.6 µM compound B; p < 0.05, “*” = 0.05, “**” = 0.01, “***” = 0.001; N = 5–10