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Transcriptome analysis of MZznf598 embryos during the MZT. (A) A scheme of RNA-Seq analysis comparing wild-type and MZznf598 embryos during the MZT. (B) Venn diagrams of mRNAs up-regulated or down-regulated at 1 hpf or 6 hpf (FDR < 0.01). The numbers of genes in each category are shown. (C) Functional enrichment analysis of biological terms associated with genes up-regulated (orange) or down-regulated (light blue) at 6 hpf. Terms that were significantly enriched (FDR < 0.01) are shown. (D) A volcano plot showing the fold change in mRNA expression between wild-type and MZznf598 embryos at 6 hpf (x-axis) and FDR (y-axis). Genes encoding C2H2-ZF (red) and other genes (gray) are shown. (E) Bar graph showing the fractions of C2H2-ZF or RING-ZF genes among all the genes (gray), genes up-regulated in MZznf598 (orange), and genes down-regulated in MZznf598 (blue) at 6 hpf. The p value was calculated by the chi-square test. (F) Cumulative distributions of fold changes in mRNA levels between MZznf598 embryos and wild-type embryos at 6 hpf. All genes (black), C2H2-ZF genes (red), and RING-ZF genes (purple) are shown. The x-axis shows the fold change, and the y-axis shows the cumulative fraction. The p values are shown on the right (Kolmogorov–Smirnov test). (G) Bar graphs summarizing the expression patterns of C2H2-ZF genes (red) and other genes (gray) up-regulated in MZznf598 embryos at 6 hpf. The numerical data underlying this figure can be found in S1 Data. hpf, hours postfertilization; MZT, maternal-to-zygotic transition.
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