FIGURE

Figure 2

ID

ZDB-FIG-241115-117

Publication

Zhao et al., 2024 - The Glutamine Synthetases Are Required for Sensory Hair Cell Formation and Auditory Function in Zebrafish

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Figure 2

The downregulation of glula results in a reduced survival rate and impacts otolith development. (A) Diagram illustrating the glula-MO binding site and potential cleavage methods. (B) Reproducible knockdown of the glula gene was successfully demonstrated through PCR experimentation. (C) Knockdown of glula leads to a significant decrease in the survival rate of zebrafish. (D) Abnormal otolith development was observed in zebrafish following glula-MO injection. (E) Statistical analysis revealed that 98% of zebrafish injected with glula-MO exhibited aberrant otolith development. Chi-squared test: ****, p < 0.0001. (F) After the injection of glula-MO, a significant decrease in hair cells was observed in otic vesicles. Dash line cycles indicate the crista hair cells. (G) Statistical analysis of the hair cell number in otic vesicles. t-test: ***, p < 0.001.

Expression Data

Gene:	GFP
Fish:	s356tTg (AB) s356tTg + MO1-glula (AB)
Knockdown Reagent:	MO1-glula
Anatomical Term:	otic vesicle hair cell
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-glula s356tTg + MO1-glula (AB)
Knockdown Reagent:	MO1-glula
Observed In:	otic vesicle hair cell otolith whole organism
Stage Range:	Prim-5 to Protruding-mouth

Phenotype Detail

Acknowledgments

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